VEGF Detection via Impedance Spectroscopy on Surface Functionalized Interdigitated Biosensor

Vascular endothelial growth factor (VEGF), a clinically important biomarker, often plays key role in angiogenesis, would healing, tumor growth, lung development, and retinal diseases. Hence, detecting quantifying VEGF is deemed medically clinical diagnosis for many In this report, simple yet highly cost-effective platform was proposed protein detection using commercially available interdigitated sensors that are surface modified to present DNA optimally capture. The dielectric characteristics between the fingers of sensor were modulated by negatively charged aptamer-VEGF capture, impedance estimated an analyzer. Impedance-spectra tests compared among pristine unmodified surfaces, functionalized monolayer aptamer-grafted surfaces order evaluate efficacy detection. From our results, sensitivity experiments as conducted showed ability detect at low concentration 5 pM (200 pg/mL). specificity further examined comparing platelet-derived factor, results confirm sensor. Finally, Nyquist plot spectra also presented help data visualization overall performance device found be suitable template smart biosensor VEGF.